A novel thermophilic glycosynthase that effects branching glycosylation

Bioorg Med Chem Lett. 2000 Feb 21;10(4):365-8. doi: 10.1016/s0960-894x(99)00700-3.

Abstract

A novel thermophilic glycosynthase that effects branching glycosylation has been obtained by mutation of the nucleophile in the active site of the glycosidase from Sulfolobus solfataricus. Two methods for the use of this mutant are reported.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • Binding Sites / genetics
  • Catalysis
  • Glucose / analogs & derivatives
  • Glucose / metabolism
  • Glycoside Hydrolases / genetics*
  • Glycoside Hydrolases / metabolism*
  • Glycosylation / drug effects
  • Hot Temperature
  • Mutagenesis, Site-Directed
  • Point Mutation
  • Sulfolobus / enzymology

Substances

  • glucosyl fluoride
  • Glycoside Hydrolases
  • Glucose