Abstract
A mini-Tn5-Hg insertion mutant derived from Pseudomonas aeruginosa W51D (W51M1) was isolated in which mini-Tn5 insertion disrupted the motR gene showing that it forms part of the cluster involved in bacterial motility and chemotaxis. Characterization of the W51M1 motility behavior, and also of a PAO1 motR::mini-Tn5-Hg mutant, suggests that the product of the motR gene is a negative regulator of bacterial motility which controls the number of flagella per cell.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / genetics*
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Bacterial Proteins / physiology
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Base Sequence
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Chemotaxis / genetics
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Chemotaxis / physiology
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DNA Transposable Elements / genetics
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Molecular Sequence Data
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Movement / physiology
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Mutagenesis, Insertional
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Mutation
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Operon
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Pseudomonas aeruginosa / genetics*
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Pseudomonas aeruginosa / physiology
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Sequence Analysis, DNA
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Sigma Factor / genetics
Substances
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Bacterial Proteins
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DNA Transposable Elements
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DNA, Bacterial
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FliA protein, Bacteria
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MotR protein, Pseudomonas aeruginosa
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Sigma Factor