Effects of pentoxifylline and protein kinase C inhibitor on phorbol ester-induced intercellular adhesion molecule-1 expression in brain microvascular endothelial cells

Zhongguo Yao Li Xue Bao. 1999 Aug;20(8):741-4.

Abstract

Aim: To study the potential roles of protein kinase C (PKC) on expression of intercellular adhesion molecule-1 (ICAM-1) in rat brain microvascular endothelial cells (RBMEC).

Methods: ICAM-1 expression in RBMEC was measured by ELISA analyses.

Results: Phorbol ester (PMA) enhanced the expression of ICAM-1 in a concentration (10-100 nmol.L-1) and time (4-16 h)-dependent manner in RBMEC. Pentoxifylline (PTX) 1-100 mumol.L-1 and the PKC inhibitor H7 5-50 mumol.L-1 prevented PMA-induced stimulation of ICAM-1 expression. At PTX 100 mumol.L-1 and H7 50 mumol.L-1, they reached maximal inhibitory effects [ICAM-1 expression (A) from (0.410 +/- 0.014) to (0.175 +/- 0.022) and (0.182 +/- 0.013), respectively; P < 0.01].

Conclusion: Activation of PKC in RBMEC is associated with increased expression of ICAM-1 in RBMEC. PTX and H7 preincubation may inhibit PKC-induced up-regulation of ICAM-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / pharmacology*
  • Animals
  • Brain / blood supply
  • Capillaries / cytology
  • Cells, Cultured
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / metabolism*
  • Female
  • Intercellular Adhesion Molecule-1 / biosynthesis*
  • Male
  • Pentoxifylline / pharmacology*
  • Protein Kinase C / antagonists & inhibitors*
  • Rats
  • Rats, Wistar
  • Tetradecanoylphorbol Acetate / antagonists & inhibitors
  • Vasodilator Agents / pharmacology

Substances

  • Vasodilator Agents
  • Intercellular Adhesion Molecule-1
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Protein Kinase C
  • Tetradecanoylphorbol Acetate
  • Pentoxifylline