Effects of capsaicin-induced sensory denervation on osteoclastic resorption in adult rats

Exp Physiol. 2000 Jan;85(1):62-6. doi: 10.1017/s0958067000019308.

Abstract

Many recent findings suggest that the nervous system has efferent effects on bone. A putative role of the sensory innervation has been assessed by using a synchronised rat model of bone resorption after treating adult animals with the neurotoxin capsaicin. Fourteen days after capsaicin treatment (50 mg kg-1) the right maxillary molars were extracted to activate a wave of resorption along the mandibular cortex. The rats were killed 4 days later (i.e. at the peak of resorption in this model), and their right mandibles were processed for histometric evaluation of resorption along the cortex and of calcitonin gene-related peptide (CGRP)- and substance P (SP)-immunoreactive (IR) fibres in the dental pulp. CGRP-IR and SP-IR fibres were significantly reduced in numbers by the capsaicin treatment (by 58 and 49%, respectively), confirming the success of sensory denervation. The resorption surface was significantly reduced (P < 0.005) versus the sham-treated animals. Although the size of the osteoclast population recruited in the site was not modified, the number of actively resorbing osteoclasts was significantly reduced (P < 0.03). However, the activity of the resorbing cells was not modified. Non-specific esterase-positive osteoclast precursors were also significantly few after capsaicin treatment. These data show that the sensory nervous system is involved in the control of bone resorption at two different levels: (1) in the recruitment of osteoclast precursors, and (2) in regulating the access of recruited cells to the bone surface.

MeSH terms

  • Animals
  • Bone Resorption / pathology
  • Bone Resorption / prevention & control*
  • Calcitonin Gene-Related Peptide / physiology
  • Capsaicin / pharmacology*
  • Denervation
  • Dental Pulp / drug effects
  • Dental Pulp / physiology
  • Immunohistochemistry
  • Male
  • Maxilla / pathology
  • Nerve Fibers / drug effects
  • Nerve Fibers / physiology
  • Neurons, Afferent / drug effects*
  • Rats
  • Rats, Wistar
  • Substance P / physiology

Substances

  • Substance P
  • Calcitonin Gene-Related Peptide
  • Capsaicin