Objective: This paper was designed to evaluate 6 short tandem repeats(STR) loci for their use in forensic personal identification.
Methods: The allele frequencies and genotype frequencies at 6 tetranucleotide STR loci were determined in a sample of 132 unrelated individuals from a Chinese population of Han nationality in Guangzhou. The PCR products were analysed on 4% denaturing PAGE and detected using fluorescently labeled primers in an automated 377 sequencer(PE). The resultant allele frequencies were compared with other population or race databases.
Results: All the 6 loci (vWA31A, TH01, F13A01,FES, TPOX, CSF1PO) met Hardy-Weinberg equilibrium. No random association of alleles among the 6 loci was noted. Except locus vWA31A, the observed heterozygosities at other 5 loci were significantly lower than those reported in Caucasian and Black population studies. The calculated DP=0.99999,PE=0.9708,pM=1.059 times 10(-5).
Conclusion: The data obtained can be used in human identity and paternity testing, and in other genetic researches and population investigation.