Employing target modifications for the investigation of liquid infrared matrix-assisted laser desorption/ionization mass spectrometry

Rapid Commun Mass Spectrom. 2000;14(2):53-60. doi: 10.1002/(SICI)1097-0231(20000130)14:2<53::AID-RCM833>3.0.CO;2-H.

Abstract

We present a new target modification for commercial ion sources to facilitate the use of liquid matrices such as glycerol and lactic acid in infrared matrix-assisted laser desorption/ionization (IR-MALDI). Benefits of this modification include increased sensitivity compared with the unmodified commercial target, mass resolution comparable to the best achievable with conventional IR-MALDI or UV-MALDI, and decreased sample volatility leading to an analysis time extended up to fivefold compared with using conventional target designs (up to 1 hour using volumes of only 250 nL).

Publication types

  • Comparative Study

MeSH terms

  • Cytochrome c Group / chemistry
  • Glycerol
  • Insulin / chemistry
  • Lactic Acid
  • Peptide Fragments / chemistry
  • Sensitivity and Specificity
  • Solutions
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Volatilization
  • alpha-Fetoproteins / chemistry

Substances

  • Cytochrome c Group
  • Insulin
  • Peptide Fragments
  • Solutions
  • alpha-Fetoproteins
  • Lactic Acid
  • Glycerol