Specific detection of Leuconostoc mesenteroides subsp. mesenteroides with DNA primers identified by randomly amplified polymorphic DNA analysis

Appl Environ Microbiol. 2000 Jan;66(1):422-4. doi: 10.1128/AEM.66.1.422-424.2000.

Abstract

Randomly amplified polymorphic DNA analysis using primer 239 (5' CTGAAGCGGA 3') was performed to characterize Leuconostoc sp. strains. All the strains of Leuconostoc mesenteroides subsp. mesenteroides (with the exception of two strains), two strains formerly identified as L. gelidum, and one strain of Leuconostoc showed a common band at about 1.1 kb. This DNA fragment was cloned and sequenced in order to verify its suitability for identifying L. mesenteroides subsp. mesenteroides strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Primers
  • DNA, Bacterial / analysis
  • Leuconostoc / classification
  • Leuconostoc / genetics
  • Leuconostoc / isolation & purification*
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Random Amplified Polymorphic DNA Technique*

Substances

  • DNA Primers
  • DNA, Bacterial

Associated data

  • GENBANK/AF124525