Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins

RNA. 1999 Dec;5(12):1597-604. doi: 10.1017/s1355838299991288.

Abstract

We have identified an apparent human homolog of the yeast Nop5/Nop58 protein. hNop5/Nop58 codes for a protein of predicted molecular weight 59.6 kDa and is 46.8% identical to Saccharomyces cerevisiae Nop5/Nop58. Immunofluorescent staining with antibodies against hNop5/Nop58 indicate that it is localized primarily to the nucleolus, and coimmunoprecipitation from nuclear extracts demonstrates that hNop5/Nop58 interacts with the box C/D family of snoRNAs. Thus, hNop5/Nop58 is a common component of the box C/D snoRNPs, and joins fibrillarin as the second such component identified and characterized in metazoans.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Nucleolus / metabolism
  • Cloning, Molecular
  • Conserved Sequence
  • HeLa Cells
  • Humans
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Nuclear Proteins / analysis
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / genetics*
  • Rats
  • Recombinant Proteins / chemistry
  • Ribonucleoproteins / analysis
  • Ribonucleoproteins / chemistry
  • Ribonucleoproteins / genetics*
  • Ribonucleoproteins, Small Nucleolar / analysis
  • Ribonucleoproteins, Small Nucleolar / chemistry
  • Ribonucleoproteins, Small Nucleolar / genetics*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • NOP58 protein, S cerevisiae
  • NOP58 protein, human
  • Nop58 protein, rat
  • Nuclear Proteins
  • Recombinant Proteins
  • Ribonucleoproteins
  • Ribonucleoproteins, Small Nucleolar
  • Saccharomyces cerevisiae Proteins

Associated data

  • GENBANK/AA148805
  • GENBANK/AA437736
  • GENBANK/U56656