Mean weighted nuclear volumes in colonic adenocarcinomas. Evaluation of prognostic significance

Anal Quant Cytol Histol. 1999 Jun;21(3):194-200.

Abstract

Objective: To investigate the correlation of stereologically estimated mean weighted nuclear volumes of tumor cell nuclei (MWNV) with other prognostic factors and survival in colonic adenocarcinomas.

Study design: The study group consisted of 42 patients with colonic adenocarcinomas who were treated by a standardized protocol and had a mean follow-up of 89 months. The point sample intercepts method was used to estimate MWNV. In addition, immunohistochemical expression of p53 and Ki-67 was evaluated semiquantitatively.

Results: The calculated nuclear volumes correlated significantly with histologic grading and with the extent of tumor progression (organ confined/nonorgan confined). There was no correlation with p53 expression or proliferative activity determined by MIB-1 positivity. No correlation could be demonstrated with sex, age or clinical outcome of the patients.

Conclusion: Assessment of MWNV in colonic adenocarcinomas does not provide additional information concerning the clinical course.

MeSH terms

  • Adenocarcinoma / chemistry
  • Adenocarcinoma / mortality
  • Adenocarcinoma / pathology*
  • Aged
  • Aged, 80 and over
  • Cell Division
  • Cell Nucleus / chemistry
  • Cell Nucleus / pathology*
  • Colonic Neoplasms / chemistry
  • Colonic Neoplasms / mortality
  • Colonic Neoplasms / pathology*
  • Evaluation Studies as Topic
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Humans
  • Image Cytometry
  • Image Processing, Computer-Assisted
  • Karyometry
  • Ki-67 Antigen / analysis
  • Male
  • Middle Aged
  • Prognosis
  • Regression Analysis
  • Survival Rate
  • Tumor Suppressor Protein p53 / analysis

Substances

  • Ki-67 Antigen
  • Tumor Suppressor Protein p53