Centrosome duplication in mammalian somatic cells requires E2F and Cdk2-cyclin A

Nat Cell Biol. 1999 Jun;1(2):88-93. doi: 10.1038/10054.

Abstract

Centrosome duplication is a key requirement for bipolar spindle formation and correct segregation of chromosomes during cell division. In a manner highly reminiscent of DNA replication, the centrosome must be duplicated once, and only once, in each cell cycle. How centrosome duplication is regulated and coordinated with other cell-cycle functions remains poorly understood. Here, we have established a centrosome duplication assay using mammalian somatic cells. We show that centrosome duplication requires the activation of E2F transcription factors and Cdk2-cyclin A activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CDC2-CDC28 Kinases*
  • CHO Cells
  • Carrier Proteins*
  • Cell Cycle / physiology*
  • Cell Cycle Proteins / metabolism
  • Centrosome / physiology*
  • Centrosome / ultrastructure*
  • Cricetinae
  • Cyclin A / metabolism*
  • Cyclin-Dependent Kinase 2
  • Cyclin-Dependent Kinases / metabolism*
  • DNA Replication*
  • DNA-Binding Proteins*
  • E2F Transcription Factors
  • Mammals
  • Protein Serine-Threonine Kinases / metabolism*
  • Recombinant Proteins / metabolism
  • Retinoblastoma-Binding Protein 1
  • Transcription Factors / metabolism*
  • Transfection

Substances

  • Carrier Proteins
  • Cell Cycle Proteins
  • Cyclin A
  • DNA-Binding Proteins
  • E2F Transcription Factors
  • Recombinant Proteins
  • Retinoblastoma-Binding Protein 1
  • Transcription Factors
  • Protein Serine-Threonine Kinases
  • CDC2-CDC28 Kinases
  • Cyclin-Dependent Kinase 2
  • Cyclin-Dependent Kinases