Induction of isoflavonoid and retrochalcone branches of the flavonoid pathway in cultured Glycyrrhiza echinata cells treated with yeast extract

Biosci Biotechnol Biochem. 1999 Sep;63(9):1618-20. doi: 10.1271/bbb.63.1618.

Abstract

Yeast extract-treated suspension cultures of a new cell line, AK-1, of Glycyrrhiza echinata were induced to produce an isoflavonoid phytoalexin (medicarpin) and metabolites of retrochalcone/flavone pathway (echinatin, licodione, and 7,4'-dihydroxyflavone). From these cells, putative full-length cDNAs encoding cytochrome P450s, (2S)-flavanone 2-hydroxylase and isoflavone 2'-hydroxylase, were cloned.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • Cytochrome P-450 Enzyme System / genetics
  • DNA, Complementary / genetics
  • DNA, Plant / genetics
  • Flavonoids / chemistry
  • Flavonoids / metabolism*
  • Glycyrrhiza / genetics
  • Glycyrrhiza / metabolism*
  • Glycyrrhiza / microbiology
  • Isoflavones / chemistry
  • Isoflavones / metabolism
  • Mixed Function Oxygenases / genetics
  • Plants, Medicinal*
  • Yeasts / metabolism

Substances

  • DNA, Complementary
  • DNA, Plant
  • Flavonoids
  • Isoflavones
  • Cytochrome P-450 Enzyme System
  • Mixed Function Oxygenases
  • cytochrome P-450 CYP81E1 (Glycyrrhiza echinata)
  • cytochrome P-450 CYP93B1 (Glycyrrhiza echinata)

Associated data

  • GENBANK/AB022732
  • GENBANK/AB022733