Local circuit neurons showing calbindin D28k-immunoreactivity in the substantia gelatinosa of the medullary dorsal horn of the rat. An immunohistochemical study combined with intracellular staining in slice preparation

Brain Res. 1999 Sep 4;840(1-2):179-83. doi: 10.1016/s0006-8993(99)01794-1.

Abstract

Calbindin D28k (CB)-immunoreactive local circuit neurons in the substantia gelatinosa of the rat medullary dorsal horn were studied in slice preparations; intracellular injection of biocytin was followed by histochemical visualization of biocytin, then CB immunohistochemistry was performed. In six neurons so far examined, overall morphology, including those of dendritic fields, axonal fields, and arborization patterns, was common.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calbindin 1
  • Calbindins
  • Histocytochemistry
  • Immunohistochemistry
  • In Vitro Techniques
  • Intracellular Membranes / metabolism
  • Lysine / analogs & derivatives
  • Male
  • Medulla Oblongata
  • Neural Pathways / cytology
  • Neural Pathways / metabolism
  • Posterior Horn Cells / cytology
  • Posterior Horn Cells / metabolism*
  • Posterior Horn Cells / physiology
  • Posterior Horn Cells / ultrastructure
  • Rats
  • Rats, Wistar
  • S100 Calcium Binding Protein G / metabolism*
  • Spinal Cord / cytology
  • Spinal Cord / metabolism*
  • Staining and Labeling
  • Substantia Gelatinosa / drug effects
  • Substantia Gelatinosa / metabolism*

Substances

  • Calb1 protein, rat
  • Calbindin 1
  • Calbindins
  • S100 Calcium Binding Protein G
  • biocytin
  • Lysine