Abstract
The plant hormone auxin is transported in a polar manner along the shoot-root axis, which requires efflux carriers such as PIN1. Asymmetric localization of PIN1 develops from a random distribution in Arabidopsis early embryogenesis. Coordinated polar localization of PIN1 is defective in gnom embryos. GNOM is a membrane-associated guanine-nucleotide exchange factor on ADP-ribosylation factor G protein (ARF GEF). Thus, GNOM-dependent vesicle trafficking may establish cell polarity, resulting in polar auxin transport.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ADP-Ribosylation Factors / metabolism*
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Arabidopsis / cytology
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Arabidopsis / embryology
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Arabidopsis / genetics
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Arabidopsis / metabolism*
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Arabidopsis Proteins*
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Biological Transport
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Brefeldin A / pharmacology
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Cell Membrane / metabolism
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Cell Polarity
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Cytosol / metabolism
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Guanine Nucleotide Exchange Factors / chemistry
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Guanine Nucleotide Exchange Factors / genetics
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Guanine Nucleotide Exchange Factors / metabolism*
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Indoleacetic Acids / metabolism*
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Membrane Proteins / metabolism*
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Membrane Transport Proteins*
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Plant Roots / growth & development
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Plant Roots / metabolism
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Protein Conformation
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Recombinant Proteins / metabolism
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Seeds / cytology
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Seeds / metabolism
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Solubility
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Subcellular Fractions / metabolism
Substances
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Arabidopsis Proteins
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Guanine Nucleotide Exchange Factors
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Indoleacetic Acids
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Membrane Proteins
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Membrane Transport Proteins
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PIN1 protein, Arabidopsis
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Recombinant Proteins
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Brefeldin A
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ADP-Ribosylation Factors