A novel chromosomal region of allelic loss, 4q32-q34, in human osteosarcomas revealed by representational difference analysis

Genes Chromosomes Cancer. 1999 Oct;26(2):115-24.

Abstract

Representational difference analysis (RDA) of a human osteosarcoma xenograft resulted in the isolation of four tumor-associated homozygously deleted DNA fragments, all originating from chromosome 4, region q32-q34. Southern blot analysis using the RDA fragments and interphase FISH analysis using PACs corresponding to these RDA fragments revealed allelic loss of the 4q32-q34 region in 17 of 27 (63%) osteosarcomas tested. These results suggest the involvement of tumor suppressor gene(s) within this chromosomal region in osteosarcoma development. The RDA fragments and corresponding PAC clones will be instrumental in the isolation of such gene(s). Genes Chromosomes Cancer 26:115-124, 1999.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Blotting, Southern
  • Chromosome Mapping
  • Chromosomes, Human, Pair 4 / genetics*
  • DNA, Neoplasm / isolation & purification
  • Humans
  • In Situ Hybridization, Fluorescence
  • Loss of Heterozygosity / genetics*
  • Mice
  • Mice, Nude
  • Nucleic Acid Hybridization
  • Osteosarcoma / genetics*
  • Transplantation, Heterologous
  • Tumor Cells, Cultured

Substances

  • DNA, Neoplasm