Experimental study on the role of mast cells in peritoneal adhesion formation

East Afr Med J. 1999 Apr;76(4):233-6.

Abstract

Objective: To investigate the potential role of mast cell stabilisation in the prevention of post-operative adhesions.

Design: Laboratory animal experiment.

Setting: University hospital, Turkey.

Subjects: Ninety Wistar albino rats.

Intervention: Under anaesthesia, a lower midline laparotomy was performed, the caecum exposed and grasped until haemorrhage occurred. The rats were divided into three groups. Group 1, 2 and 3 were intra-peritoneally administered 1 ml of saline, disodium cromoglycate 5 mg/kg in 1 ml of saline and 10 mg/kg in 1 ml of saline, respectively thirty minutes prior to laparotomy and immediately subsequent to abdominal closure. They were later sacrificed, laparotomy repeated and the presence and extent of intraabdominal adhesions evaluated.

Results: Adhesion scores were best in the high disodium cromoglycate dose group of rats (p < 0.05) and the number of degranulated mast cells was significantly low in this group (p < 0.05).

Conclusion: Disodium cromoglycate may be an effective agent for attenuating adhesion formation when administered in suitable doses.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / therapeutic use
  • Cromolyn Sodium / therapeutic use
  • Female
  • Male
  • Mast Cells / drug effects
  • Mast Cells / physiology*
  • Peritoneal Diseases / prevention & control*
  • Postoperative Complications / prevention & control*
  • Rats
  • Rats, Wistar
  • Statistics, Nonparametric
  • Tissue Adhesions / prevention & control*

Substances

  • Anti-Inflammatory Agents
  • Cromolyn Sodium