Characterisation of Streptococcus thermophilus CNRZ1205 and its cured and re-lysogenised derivatives

FEMS Microbiol Lett. 1999 Jul 15;176(2):503-10. doi: 10.1111/j.1574-6968.1999.tb13704.x.

Abstract

Streptococcus thermophilus CNRZ1205 is the lysogenic host for the temperate phage phi O1205. A derivative of CNRZ1205 was isolated which was cured of phi O1205 and this strain was used to construct a re-lysogenised derivative. Pulse field gel electrophoresis and sequencing of the attachment site regions confirmed that excision and re-integration of the phage was a site-specific event. Interestingly, cells from the cured, as well as its re-lysogenised derivative, were found to have a very long chain length.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Transposable Elements
  • Electrophoresis, Gel, Pulsed-Field
  • Lysogeny
  • Streptococcus / genetics
  • Streptococcus / isolation & purification
  • Streptococcus / physiology*
  • Streptococcus Phages / physiology*

Substances

  • DNA Transposable Elements