A mutational study of a Diels-Alderase catalytic antibody

F E Romesberg; P G Schultz

doi:10.1016/s0960-894x(99)00281-4

A mutational study of a Diels-Alderase catalytic antibody

Bioorg Med Chem Lett. 1999 Jul 5;9(13):1741-4. doi: 10.1016/s0960-894x(99)00281-4.

Authors

F E Romesberg¹, P G Schultz

Affiliation

¹ Howard Hughes Medical Institute and the Department of Chemistry, University of California, Berkeley 94720-1460, USA.

PMID: 10406634
DOI: 10.1016/s0960-894x(99)00281-4

Abstract

Site-directed mutagenesis was used to probe the mechanism of antibody 39-A11, which catalyzes the Diels-Alder reaction of substrate 1 and 2 to give cycloadduct 3. Mutations that lead to improved packing interactions with the diene afford an order of magnitude increase in catalytic activity.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Antibodies, Catalytic / chemistry*
Crystallography, X-Ray
Kinetics
Models, Molecular
Mutagenesis, Site-Directed

Substances

Antibodies, Catalytic

Grants and funding

AI24695/AI/NIAID NIH HHS/United States