We assessed the prevalence of licit (e.g. alcohol) and illicit (e.g. cocaine) drug use, as well as prescription (e.g. tranquillizers) and over-the-counter medications (e.g. analgesics), in a regional sample of female nurses. Surveys were mailed to a random sample of 4000 nurses in Western New York. The survey focused on lifetime and current use of substances, negative consequences of alcohol consumption and dependence. Three mailings resulted in a return of 2400 (60%) surveys, of which 1951 (49%) were usable. We examined lifetime and current use in each of the four classes of substances in the context of nursing related-factors (e.g. type of nurse, nursing specialty, work setting) and demographic characteristics (e.g. age, marital status). There were significant differences within each of the different groupings. Lifetime experience of negative consequences were relatively rare and few nurses reported dependence on substances other than tobacco and caffeine.