The lipoprotein lipase HindIII polymorphism: association with total cholesterol and LDL-cholesterol, but not with HDL and triglycerides in 342 females

Clin Chem. 1999 Jul;45(7):963-8.

Abstract

Background: Lipoprotein lipase (LPL) is the rate-limiting enzyme in the hydrolysis of core triglycerides in chylomicrons and VLDL.

Methods: We investigated the association between the HindIII polymorphism of the LPL gene and fasting glucose, lipid, and lipoprotein concentrations in 683 Caucasians. We first stabilized the study subjects, using an 8-day diet and exercise intervention program before obtaining blood samples. The use of this standardization period reduced the variance of all glucose and lipid concentrations.

Results: In our study, the HindIII allele frequencies for females and males were 0.29 and 0.34 for H- and 0.71 and 0.66 for H+, respectively. We found in females, but not in males, a significant association between the HindIII genotype and total cholesterol (P = 0.007) and LDL-cholesterol (P = 0.018), with females homozygous for the rare H- allele having the lowest, heterozygotes (H-/+) having intermediate, and women homozygous for the common H+ allele having the highest of each of these lipid traits. With regard to triglycerides, HDL-cholesterol, and glucose, no significant effect of the HindIII genotype was noted in either gender.

Conclusions: These results suggest that in a gender-specific manner, the rare LPL HindIII H- allele has a cholesterol-lowering and, therefore, potentially cardioprotective effect compared with the common H+ allele.

Publication types

  • Clinical Trial

MeSH terms

  • Alleles
  • Cholesterol / blood*
  • Cholesterol, HDL / blood*
  • Cholesterol, LDL / blood*
  • Deoxyribonuclease HindIII
  • Diet
  • Exercise
  • Female
  • Humans
  • Lipoprotein Lipase / genetics*
  • Male
  • Middle Aged
  • Phenotype
  • Polymorphism, Restriction Fragment Length
  • Sex Factors
  • Triglycerides / blood*

Substances

  • Cholesterol, HDL
  • Cholesterol, LDL
  • Triglycerides
  • Cholesterol
  • Lipoprotein Lipase
  • Deoxyribonuclease HindIII