In pyramidal neurons from rat visual cortex slices, bath-application of NT-4 (20 ng/ml) did not much affected the baseline calcium signal, but did enhance calcium signals elicited by injections of depolarizing currents (+0.5 nA, 1 s). This enhancing effect of NT-4 was abolished by co-applying K252a. With ryanodine injected intracellularly, the effect of NT-4 was significantly reduced, suggesting an involvement of intracellular calcium release in this NT-4-induced enhancement of calcium transient.
Copyright 1999 Elsevier Science B.V.