Objective: To study the correlation between t(14;18) chromosomal translocation and the occurrence of hepatocarcinoma.
Methods: bcl-2/JH fusion gene in 54 cases of hepatocarcinoma including 40 cases of hepatocellular carcinoma(HCC) and 14 cases of cholangicellular carcinoma (CCC) were investigated using seminested in situ PCR.
Results: Bcl-2/JH fusion gene was present in 25% (10/40) HCC and negative in CCC.
Conclusion: These findings indicated that t(14;18) chromosomal translocation might play a role in the pathogenesis of HCC but seemed to bear no relation with CCC. mbr and mcr are the two important breakpoints of bcl-2 gene in HCC.