Cell orientation induced by extracellular signals

Cell Biochem Biophys. 1999;30(2):167-92. doi: 10.1007/BF02738066.

Abstract

Cells like fibroblasts and osteoblasts are oriented by different extracellular guiding signals like an electric field, a bent surface, and a periodically stretched surface. An automatic controller is responsible for the cell alignment. The controller contains both a deterministic and a stochastic signal. The following machine properties were determined: (1) The angle dependence of the cellular signal transformer is cos 2(psi 0 - psi). (2) The set point of the automatic controller is psi 0 = +/- 90 degrees. The cells like to orient their long axis perpendicular to the direction of the applied guiding signal. (3) The signal transformer measures the extracellular signal in a quadratic fashion. The cells cannot register the sign of the guiding field. (4) The stochastic signal in the automatic controller can be quantified by a characteristic time (approximately 130 min for fibroblasts). (5) The extracellular signal is registered in cell-made standards (ratio of the deterministic and stochastic signal equals one): 0.3 +/- 0.05 V/mm for human fibroblasts (electric field) and 85 +/- 3 microns for human fibroblasts and osteoblasts (cyclindrically bent surface). (6) The lag-time in the signal transduction system of fibroblasts is approximately 4 min.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Polarity / physiology*
  • Electrophysiology / instrumentation
  • Electrophysiology / methods
  • Fibroblasts / physiology
  • Humans
  • Kinetics
  • Models, Theoretical
  • Osteoblasts / physiology
  • Signal Transduction / physiology*
  • Stochastic Processes
  • Time Factors