Objective: To study if there exists superinfection and multiple infection in the patients with hepatitis G virus (HCV) infection.
Methods: Reverse transcription polymerase chain reaction (RT-PCR) technique in non-structural gene 3 (NS3) region of HGV was used to detect HGV RNA in serially diluted quality control sera and HGV-C reference panel of samples provided by Abbott Co. Ltd, in 90 cases with positive hepatitis C virus (HCV) RNA, and 12 blood donors with super-infection of hepatitis B virus (HBV) and hepatitis C virus (HCV).
Results: Serially diluted quality control sera showed positive for HGV RNA at dilutions of 10(-1) to 10(-5), but negative at dilutions of 10(-6) to 10(-8). HGV RNA was positive in two samples of HGV-C serum provided by Abbott Co. HGV RNA was positive in eight of the 90 cases with positive HCV RNA and in four of the 12 cases with super-infection with HCV and HBV.
Conclusion: There are not only super-infection of HBV and HCV, but also multiple infection.