Abstract
The serotonin transporter (5-HTT) plays a key-role in the control of serotoninergic neurotransmission and is the target of some antidepressants. Possible adaptive changes in brain 5-HT2A receptors were investigated in knock-out mice that do not express the 5-HTT. Autoradiographic labeling of these receptors by the selective antagonist [3H]MDL 100,907 and saturation experiments with cortical membranes revealed: (1) a new localization of these receptors in the external field of striatum (possibly in striosomes); (2) regional variations in adaptive changes in the density of 5-HT2A receptors in 5-HT(-/-) mutants (-30-40% in the claustrum, cerebral cortex and lateral striatum; no significant change in the striatum core) as compared to wild-type mice.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptation, Physiological
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Animals
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Binding Sites
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Carrier Proteins / genetics
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Carrier Proteins / physiology*
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Cell Membrane / metabolism
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Cerebral Cortex / cytology
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Cerebral Cortex / metabolism*
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Down-Regulation
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Fluorobenzenes / metabolism
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Heterozygote
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Homozygote
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / physiology*
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Membrane Transport Proteins*
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Mutation
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Nerve Tissue Proteins*
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Piperidines / metabolism
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Receptor, Serotonin, 5-HT2A
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Receptors, Serotonin / metabolism*
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Serotonin Antagonists / metabolism
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Serotonin Plasma Membrane Transport Proteins
Substances
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Carrier Proteins
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Fluorobenzenes
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Membrane Glycoproteins
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Membrane Transport Proteins
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Nerve Tissue Proteins
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Piperidines
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Receptor, Serotonin, 5-HT2A
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Receptors, Serotonin
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Serotonin Antagonists
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Serotonin Plasma Membrane Transport Proteins
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Slc6a4 protein, mouse
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volinanserin