Abstract
GerE is the latest-acting of a series of factors which regulate gene expression in the mother cell during sporulation in Bacillus. The gene encoding GerE has been cloned from B. subtilis and overexpressed in Escherichia coli. Purified GerE has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol as a precipitant. The small plate-like crystals belong to the monoclinic space group C2 and diffract beyond 2.2 A resolution with a synchrotron radiation X-ray source.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Bacillus subtilis / chemistry*
-
Bacillus subtilis / physiology
-
Bacterial Proteins / chemistry*
-
Cloning, Molecular
-
Crystallization
-
Crystallography, X-Ray
-
Electrophoresis, Polyacrylamide Gel
-
Recombinant Fusion Proteins / chemistry
-
Recombinant Fusion Proteins / isolation & purification
-
Sigma Factor*
-
Spores, Bacterial
-
Transcription Factors*
Substances
-
Bacterial Proteins
-
Recombinant Fusion Proteins
-
Sigma Factor
-
Transcription Factors
-
spoIIR protein, Bacillus subtilis
-
spore-specific proteins, Bacillus