Crystallization and preliminary X-ray crystallographic analysis of the unusual ferritin from Listeria innocua

A Ilari; C Savino; S Stefanini; E Chiancone; D Tsernoglou

doi:10.1107/s0907444998012177

Crystallization and preliminary X-ray crystallographic analysis of the unusual ferritin from Listeria innocua

Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):552-3. doi: 10.1107/s0907444998012177.

Authors

A Ilari¹, C Savino, S Stefanini, E Chiancone, D Tsernoglou

Affiliation

¹ CNR Center of Molecular Biology, Department of Biochemical Sciences 'A. Rossi Fanelli', University of Rome 'La Sapienza', Piazzale Aldo Moro, 5, 00185 Rome, Italy.

PMID: 10089376
DOI: 10.1107/s0907444998012177

Abstract

Single crystals of ferritin extracted from Listeria innocua have been obtained by the vapour-diffusion method using PEG 1000 as precipitant. The crystals are orthorhombic, space group P212121, with unit-cell dimensions a = 87.7, b = 137.5, c = 173.1 A. The crystals diffract to 2.9 A resolution on a rotating-anode X-ray source and to 2.35 A resolution on a synchrotron X-ray source. The asymmetric unit contains one molecule formed by 12 subunits, corresponding to a packing density of 2.41 A3 Da-1

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crystallization
Crystallography, X-Ray
Ferritins / chemistry*
Listeria / chemistry*
Protein Conformation

Substances

Ferritins